Ity to ET-I. In unique we hypothesized that estrogens up-regulate some

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SCHUPPE1 Center for In vitro Fertilization (C/F), 2 Center of Dermatology and Andrology, Justus Liebig University, Giessen, Germany, 3 Department of Medical Biosciences, University from the Western Cape, Bellville, South Africa (Hans-Christian. phagocytosis by mononuclear cells in the ejaculate : a marker of epididimydis pathology ? A quantitative ultrastructural analysisObjective : The fertilization potential of spermatozoa iscritically dependent on maturation inside the epididymis. One of the crucial options is progressive motility, which can be gradually acquired throughout the epididymal transit. Cellular and molecular mechanisms underlying epididymal sperm maturation, nevertheless, have not however been elucidated in detail. So that you can investigate the interaction amongst epididymal epithelium and spermatozoa in the course of this process, we developed a coculture program of epididymal epithelium and spermatozoa in a defined, serum-free culture system.F. PELLICCIONE 1, G. CORDESCHI1, R. MIHALCA 1, M. BOCCHIO1, S. NECOZIONE2, S. FRANCAVILLAChairs of I Andrology and 2 Epidemiology, University of L'Aquila, Italy Presenting author e-mail : Hyperlink "mailto :" emanuelepe@jumpy.itMaterial and Approaches : Primary cell cultures of epithelialcells obtained from caput, corpus and cauda epididymis were grown in culture medium (RPMI 1640) supplemented with 5 fetal bovi.Ity to ET-I. In particular we hypothesized that estrogens up-regulate some contractile effectors, for Ferulic acid (sodium) instance RhoA/Rho-kinase pathway, downstream for the ET-1 receptors. Design and style and Solutions : To investigate the effect of changing endocrine milieu on RhoA/Rho-kinase pathway, we induced hypogonadism (hypo) in rabbits having a single administration of two.9 mg/Kg of a long-acting GnRH analog, triptorelin, and we replaced hypo rabbits with distinct sex steroids (Testosterone, T 30 mg/Kg weekly or estradiol valerate, E2, three.3mg/Kg weekly).Final results : Just after eight weeks from GnRH analog administration, T plasma levels were decreased plus the relaxant effect on the Rho-kinase inhibitor, Y27632 on ET-1 pre-contracted epididymal strips, was considerably decreased. T administration restored T plasma levels, but not Y27632 sensitivity in the epididymal strips. E2 not simply absolutely restored Y27632 responsiveness but even amplified it, as indicating that the RhoA/Rho-kinase calcium sensitizing pathway is up-regulated by E2. Accordingly, RT-PCR studies indicate that Rho kinase gene was strongly induced by E2 but not by T. To confirm irrespective of whether endogenous estradiol is involved in the regulation of Y27632 responsiveness, we treated intact rabbits with an aromatase inhibitor, letrozole (1.25 mg/day) for 21 days. Blocking aromatase activity abolished Y27632 responsiveness in rabbit epididymis. Conclusions : Our results help the hypothesis that epididymis is a male target for E2, which regulates its motility tuning up contractile hormones and neighborhood peptides responsiveness by escalating RhoA/Rho-kinase signalling and therefore calcium sensitivity.POAn in vitro model for epididymal sperm maturationepididymis with bovine caput spermatozoa is able to trigger the development of worldwide and progressive motility. In contrast to unconditioned serum-free medium, the epididymal environment maintains sperm vitality and motility for no less than 72 hours supporting the important part of PubMed ID: epididymal cell-cell interactions for sperm maturation.C. PubMed ID: MEHNERT1, J. FECHNER2, T. STALF 1, H.R.