A Comparative Database (Broad Institute, Cambridge, USA) the MTT3 DNA sequence
As in a previous study using an in silico analysis , we identified several conserved motifs in both 5' and 3'UTR regions from the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28878015 new isolated cDNA molecules, which may be related to the regulation of their gene expression and/or transcript Adapt to a variety of environmental changes. Several cellular stresses use processing. BMC Genomics (2016) 17:Page 14 ofTable 5 Conserved motifs described in 3' and 5'UTR regions of Tetrahymena CuMT genesa New CuMT genes described in this paper. TCA and TGA trinucleotides are highlighted in blue and yellow to facilitate motif sequence comparisons and identifications of the AP-1 binding related element (TGANTCA).A Comparative Database (Broad Institute, Cambridge, USA) the MTT3 DNA sequence from T. malaccensis (registered as EIA_07390.2 hypothetical protein) shows a putative intron (60 nucleotides). After a more detailed analysis, we detected that the putative intron corresponds to the amino acid sequence of the second type 1 submodule from the second module, sequence that is quite conserved in almost all Tetrahymena CdMTs. The nucleotide sequence identified as an intron (EIA_07390.2) starts in GT and ends in AG (with a 68.3 A + T), and therefore, it might be assumed that it would coincide with the ciliate consensus intron ends (5'GTAAG/TAG 3'). A similar situation in which no introns are observed is detected in TamerMTT1 [KU052681] and TpatMTT1 [KU167652] cDNAs. The GT pair corresponds to a Cys residue (tgt codon), while AG pair corresponds to Glu (E) residue (gag codon). The same occurs in the TamerMTT1 and TpatMTT1 CdMT sequences, but not in the rest of the Tetrahymena CdMTs, because the majority codon used for Glu residues is gaa. In addition, the A + T content of these regions has very similar values to the complete ORF sequences from all CdMTs reported to date (an average of 60.5 ). All of this corroborates that the putative intron reported in a CdMT from T. malaccensis (EIA_07390.2) is not a real intron but rather an error. In general, MT PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25432023 genes are mainly regulated at transcriptional level . As in a previous study using an in silico analysis , we identified several conserved motifs in both 5' and 3'UTR regions from the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28878015 new isolated cDNA molecules, which may be related to the regulation of their gene expression and/or transcript processing. In the 5'UTR region or putative promoter we identified two types of motifs (Tables 4 and 5): a TATA box (TAATAA) with an average number of 3 motifs/ cDNA in both Cd- and CuMTs, and motifs similar to MTCM1  with an average number of 5 motifs/ cDNA in CdMTs or 3 in CuMTs. This MTCM1 motif was identified in almost all Tetrahymena MT promoters (Tables 4 and 5), so these sequences might play an important role in the gene expression regulation of these MTs. Most MTCM1 motifs include the consensus sequence TGA(N)TCA or similar (where "N" means any nucleotide), which recalls to the sequence (TGA(G/ C)TCA) binding the eukaryotic AP-1 transcription factors . In Saccharomyces cerevisiae an AP-de Francisco et al. BMC Genomics (2016) 17:Page 13 ofTable 4 Conserved motifs detected in 3' and 5' UTR regions of Tetrahymena CdMT genesa New CdMT genes reported in this study.